1GZY
Human Insulin-like growth factor; In-house data
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 30.723, 69.282, 64.528 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.000 * - 2.540 |
R-factor | 0.218 |
Rwork | 0.213 |
R-free | 0.27200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ins |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 * | 2.070 |
High resolution limit [Å] | 2.540 * | 2.540 * |
Rmerge | 0.086 * | 0.331 * |
Total number of observations | 8654 * | |
Number of reflections | 2065 * | |
<I/σ(I)> | 18 | 12 |
Completeness [%] | 98.1 * | 95 * |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | THE PROTEIN WAS CRYSTALLIZED BY THE HANGING DROP METHOD IN WHICH DROPS WERE COMPOSED OF VARIOUS RATIOS OF HIGF-I AT 7MG/ML (IN H2O) WITH RESERVOIR SOLUTION CONSISTING OF 0.1M TRIS.HCL PH 7.5, 12-15% (W/V) PEG 2K AND 5MM SB12 DETERGENT. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | in water |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.5 |
3 | 1 | reservoir | PEG2000MME | 12-15 (%(w/v)) | |
4 | 1 | reservoir | SB12 detergent | 5 (mM) |