1GZX
Oxy T State Haemoglobin - Oxygen bound at all four haems
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 277.15 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 97.050, 99.500, 66.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.195 |
Rwork | 0.199 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDN ENTRY 1HGA |
RMSD bond length | 0.018 * |
RMSD bond angle | 0.058 * |
Data reduction software | MOSFLM |
Data scaling software | ROTAVATA |
Phasing software | PROLSQ |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.090 | 0.309 |
Total number of observations | 255870 * | |
Number of reflections | 35441 | |
<I/σ(I)> | 6.1 | 2.3 |
Completeness [%] | 95.0 | 85 |
Redundancy | 4.5 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7.2 | 4 * | BATCH CRYSTALLISATION, OXYHB (75-90 MG/ML) IN 50MM POTASSIUM PHOSPHATE (PH 7.2), 22-28% PEG 8000 AND A 5 MOLAR EXCESS OF THE ALLOSTERIC EFFECTOR IHP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 75-90 (mg/ml) | |
2 | 1 | 1 | potassium phosphate | 50 (mM) | pH7.2 |
3 | 1 | 1 | PEG8000 | 22-28 (%(w/v)) |