1GZW

X-RAY CRYSTAL STRUCTURE OF HUMAN GALECTIN-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-03-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	36.979, 88.805, 93.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.410 - 1.700
R-factor	0.216
Rwork	0.216
R-free	0.22800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1slt
RMSD bond length	0.005
RMSD bond angle	26.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.410	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.049	0.337
Total number of observations	209342 *
Number of reflections	34966
<I/σ(I)>	12.2	5.8
Completeness [%]	96.1	91.4
Redundancy	3.9	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7 *		CRYSTALS WERE OBTAINED IN SITTING DROPS BY MIXING EQUAL VOLUMES OF THE PROTEIN SOLUTION (10 MG/ML) AND THE PRECIPITATING BUFFER (2M AMMONIUM SULPHATE AND 1% BETA-MERCAPTO ETHANOL, PH 5.0)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2 (M)
2	1	reservoir	beta-mercaptoethanol	1 (%)	pH5.6
3	1	drop	sodium potassium phosphate	20 (mM)
4	1	drop	lactose	5 (mM)	pH7.0
5	1	drop	protein	10 (mg/ml)