1GZQ
CD1b in complex with Phophatidylinositol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-15 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.881, 176.998, 75.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.300 - 2.260 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIALLY REFINED STRUCTURE OF CD1B-GM2 COMPLEX |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.420 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.051 | 0.206 |
| Total number of observations | 337867 * | |
| Number of reflections | 27623 * | |
| <I/σ(I)> | 39.1 | 15.8 |
| Completeness [%] | 92.3 | 89.8 |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.1 | 20 * | 20 DEGREES C, 2UL OF PROTEIN + 1UL OF MOTHER LIQUOR, 0.2M LITHIUM NITRATE, 20% W/V POLYETHYLENE GLYCOL 3350, PH 7.1 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | lithium nitrate | 0.2 (M) | |
| 2 | 1 | reservoir | EG3350 | 20 (%(w/v)) | pH7.1 |
| 3 | 1 | drop | protein | 5 (mg/ml) |
