1GZM

Structure of Bovine Rhodopsin in a Trigonal Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-15
Detector	MARRESEARCH
Spacegroup name	P 31
Unit cell lengths	103.820, 103.820, 76.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.000 - 2.650
R-factor	0.2015
Rwork	0.202
R-free	0.23500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f88
RMSD bond length	0.008
RMSD bond angle	1.293 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.790
High resolution limit [Å]	2.650	2.650
Rmerge	0.119	0.434
Number of reflections	26026
<I/σ(I)>	11	1.4
Completeness [%]	97.0	86.1 *
Redundancy	4.4	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	18 *	Edwards, P., (2004) J. Mol. Biol., 343, 1439. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir		0.6-1.1 (M)
3	1	reservoir	PEG8000	1.6 (%)
4	1	reservoir	glycerol	20 (%(v/v))