1GZM
Structure of Bovine Rhodopsin in a Trigonal Crystal Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-15 |
Detector | MARRESEARCH |
Spacegroup name | P 31 |
Unit cell lengths | 103.820, 103.820, 76.590 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.000 - 2.650 |
R-factor | 0.2015 |
Rwork | 0.202 |
R-free | 0.23500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f88 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.293 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.119 | 0.434 |
Number of reflections | 26026 | |
<I/σ(I)> | 11 | 1.4 |
Completeness [%] | 97.0 | 86.1 * |
Redundancy | 4.4 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 18 * | Edwards, P., (2004) J. Mol. Biol., 343, 1439. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | 0.6-1.1 (M) | ||
3 | 1 | reservoir | PEG8000 | 1.6 (%) | |
4 | 1 | reservoir | glycerol | 20 (%(v/v)) |