1GZJ

Structure of Thermoascus aurantiacus family 5 endoglucanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	293
Spacegroup name	P 21 21 21
Unit cell lengths	76.510, 85.850, 89.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.940 - 1.620
R-factor	0.159
Rwork	0.159
R-free	0.17700
Structure solution method	MIRAS
RMSD bond length	0.005
RMSD bond angle	1.250 *
Data reduction software	DENZO
Data scaling software	Agrovata
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.350	1.680
High resolution limit [Å]	1.620	1.620
Rmerge	0.056	0.289
Number of reflections	60922
Completeness [%]	81.6	83.9
Redundancy	5.3	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5 *	291 *	Loleggio, L., (1997) Acta Crystallogr.,Sect.D, 53, 599. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12.3 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
3	1	reservoir	ammonium sulfate	2.0 (M)