1GZI

CRYSTAL STRUCTURE OF TYPE III ANTIFREEZE PROTEIN FROM OCEAN POUT, AT 1.8 ANGSTROM RESOLUTION

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-07-18
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	32.550, 39.300, 45.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.800
Rwork	0.174
R-free	0.23300
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	0.029
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.065	0.208
Number of reflections	5155
<I/σ(I)>	6.3	3.2
Completeness [%]	99.7	100
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		50% AMMONIUM SULFATE, 0.1M SODIUM ACETATE, PH 5.6