1GZC

High-Resolution crystal structure of Erythrina cristagalli lectin in complex with lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-10-15
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	81.800, 81.800, 126.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.900 - 1.600 *
R-factor	0.208
Rwork	0.208
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ax1
RMSD bond length	0.018
RMSD bond angle	1.900
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.800	1.640
High resolution limit [Å]	1.600 *	1.580
Rmerge	0.053	0.608
Total number of observations	551905 *
Number of reflections	58614	6065 *
<I/σ(I)>	20.9	2.3
Completeness [%]	99.5	99.4
Redundancy	9.4	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	lactose	13 (%)
2	1	drop	HEPES	20 (mM)
3	1	drop		100 (mM)
4	1	drop	protein	10 (mg/ml)
5	1	reservoir	ammonium sulfate	2 (M)
6	1	reservoir	Tris-HCl	0.1 (M)	pH8.0