1GZ9
High-Resolution Crystal Structure of Erythrina cristagalli Lectin in Complex with 2'-alpha-L-Fucosyllactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-01-15 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 80.900, 80.900, 125.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.370 - 1.700 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ECL LACTOSE COMPLEX |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.980 * |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.067 | 0.693 * |
Total number of observations | 429936 * | |
Number of reflections | 46349 | 7167 * |
<I/σ(I)> | 17.5 | 2.6 |
Completeness [%] | 99.3 | 99.2 |
Redundancy | 9.3 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | used macroseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | lactose | 13 (%) | |
2 | 1 | drop | HEPES | 20 (mM) | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | reservoir | ammonium sulfate | 2 (M) | |
6 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |