1GYU
Gamma-adaptin appendage domain from clathrin adaptor AP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.090, 54.820, 68.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.640 - 1.810 |
| R-factor | 0.18 |
| Rwork | 0.175 |
| R-free | 0.22300 |
| Structure solution method | MIR |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.062 * | 0.242 |
| Number of reflections | 11924 | |
| <I/σ(I)> | 15 | 5 |
| Completeness [%] | 98.7 | 92.2 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 50MM HEPES PH7.4, 0.9M NA/K TARTRATE, 20% W/V GLYCEROL, pH 7.40 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 5 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 4 (mM) | |
| 4 | 1 | drop | protein | 5 (mg/ml) | |
| 5 | 1 | reservoir | HEPES | 50 (mM) | pH7.4 |
| 6 | 1 | reservoir | sodium acetate | 0.9 (M) | |
| 7 | 1 | reservoir | glycerol | 20 (%(w/v)) |
