1GYO
Crystal structure of the di-tetraheme cytochrome c3 from Desulfovibrio gigas at 1.2 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1997-05-15 |
Detector | MARRESEARCH |
Spacegroup name | P 31 |
Unit cell lengths | 56.670, 56.670, 94.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.500 - 1.200 |
R-factor | 0.124 * |
R-free | 0.14800 * |
Structure solution method | DIRECT METHODS |
RMSD bond length | 0.014 |
RMSD bond angle | 0.032 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.440 | 1.210 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.097 | 0.437 |
Number of reflections | 104411 | |
<I/σ(I)> | 16.3 | 2.8 |
Completeness [%] | 98.7 | 95.6 |
Redundancy | 4.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | SALTING OUT FROM A PROTEIN SOLUTION IN TRIS/MALEATE PH=6.5, pH 6.50 |