1GYL
INVOLVEMENT OF TYR24 AND TRP108 IN SUBSTRATE BINDING AND SUBSTRATE SPECIFICITY OF GLYCOLATE OXIDASE
Experimental procedure
Spacegroup name | P 4 2 2 |
Unit cell lengths | 145.500, 145.500, 100.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.500 * - 3.000 |
R-factor | 0.254 |
Rwork | 0.254 |
RMSD bond length | 0.016 |
RMSD bond angle | 2.590 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.600 * | |
High resolution limit [Å] | 3.000 * | 3.000 * |
Rmerge | 0.092 * | |
Total number of observations | 41999 * | |
Number of reflections | 18421 | |
Completeness [%] | 82.7 | 71.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 1 (M) | |
3 | 1 | reservoir | octyl pyranoside | 0.4 (%) | |
4 | 1 | reservoir | imidazol | 100 (mM) |