1GYE
Structure of Cellvibrio cellulosa alpha-L-arabinanase complexed with Arabinohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 90.605, 90.605, 177.554 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.24000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE STRUCTURE SOLVED PREVIOUSLY |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 | 0.447 |
| Number of reflections | 15610 | |
| <I/σ(I)> | 30.1 | 6.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.5 * | 10.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | 10% PEG8000, 100MM TRIS-HCL PH8.0, 20% GLYCEROL, 100MM ARABINOTRIOSE, pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 10 (%(w/v)) | |
| 2 | 1 | 1 | Tris-HCl | 100 (mM) | pH8.0 |
| 3 | 1 | 1 | glycerol | 20 (%(v/v)) | |
| 4 | 1 | 1 | protein | 20 (mg/ml) |
