1GYC
CRYSTAL STRUCTURE DETERMINATION AT ROOM TEMPERATURE OF A LACCASE FROM TRAMETES VERSICOLOR IN ITS OXIDISED FORM CONTAINING A FULL COMPLEMENT OF COPPER IONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.681, 84.977, 91.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.900 |
| Rwork | 0.159 |
| R-free | 0.20900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a65 |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 0.026 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.900 | 1.900 * |
| Rmerge | 0.063 | 0.380 * |
| Number of reflections | 52154 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 99.7 * | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | Antorini, M., (2002) Biochim.Biophys.Acta, 1594, 109. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 20 (%) | |
| 2 | 1 | reservoir | isopropanol | 20 (%) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 4 | 1 | drop | sodium acetate | 25 (mM) | pH5. |
| 5 | 1 | drop | protein | 10 (mg/ml) |
