1GYC

CRYSTAL STRUCTURE DETERMINATION AT ROOM TEMPERATURE OF A LACCASE FROM TRAMETES VERSICOLOR IN ITS OXIDISED FORM CONTAINING A FULL COMPLEMENT OF COPPER IONS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1999-04-15
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	83.681, 84.977, 91.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.900
Rwork	0.159
R-free	0.20900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a65
RMSD bond length	0.011 *
RMSD bond angle	0.026 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900	1.900 *
Rmerge	0.063	0.380 *
Number of reflections	52154
<I/σ(I)>	15.1
Completeness [%]	99.7 *
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6		Antorini, M., (2002) Biochim.Biophys.Acta, 1594, 109. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%)
2	1	reservoir	isopropanol	20 (%)
3	1	reservoir	sodium citrate	100 (mM)	pH5.6
4	1	drop	sodium acetate	25 (mM)	pH5.
5	1	drop	protein	10 (mg/ml)