1GYB

N77Y point mutant of yNTF2 bound to FxFG nucleoporin repeat

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	58.840, 84.060, 61.790
Unit cell angles	90.00, 116.00, 90.00

Refinement procedure

Resolution	40.000 - 1.900
R-factor	0.197
Rwork	0.197
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.400 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	0.257
Total number of observations	266201 *
Number of reflections	39224
<I/σ(I)>	6.9	2.7
Completeness [%]	92.4	88.3
Redundancy	3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	18 *	100MM AMMONIUM ACETATE PH 6.5, 1.6M AMMONIUM SULPHATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium acetate	50 (mM)	pH6.5
2	1	drop	ammonium sulfate	0.8 (M)
3	1	drop	protein	3.5 (mg/ml)
4	1	drop	FF1	6.25 (M)
5	1	reservoir	ammonium acetate	100 (mM)	pH6.5
6	1	reservoir	ammonium sulfate	1.52 (M)