1GY2
Crystal structure of Met148Leu rusticyanin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.450, 61.430, 53.470 |
| Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
| Resolution | 53.400 - 1.820 |
| R-factor | 0.183 * |
| Rwork | 0.183 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rcy |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 2.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.400 | 1.840 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.066 | 0.345 |
| Total number of observations | 206007 * | |
| Number of reflections | 25330 | |
| <I/σ(I)> | 15.1 | 3 |
| Completeness [%] | 92.2 | 94 |
| Redundancy | 8.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 3.8 * | 4 * | 30% PEG 8000, 50 MM CITRIC ACID PH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | sulphuric acid | 10 (mM) | pH3.8 |
| 3 | 1 | reservoir | PEG8000 | 30 (%) | |
| 4 | 1 | reservoir | citric acid | 50 (mM) | pH5.0 |
