1GXU

Hydrogenase Maturation Protein HypF "acylphosphatase-like" N-terminal domain (HypF-ACP) in complex with a substrate. Crystal grown in the presence of carbamoylphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Spacegroup name	H 3 2
Unit cell lengths	57.994, 57.994, 156.414
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.270
R-factor	0.133
Rwork	0.131
R-free	0.16130 *
Structure solution method	OTHER
RMSD bond length	0.019 *
RMSD bond angle	1.881 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.270
Rmerge	0.027
Total number of observations	292257 *
Number of reflections	27134
<I/σ(I)>	35.7
Completeness [%]	99.9
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5 *		pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	30.0 (%(w/v))
2	1	reservoir	sodium acetate	100 (mM)	pH5.5
3	1	drop	protein	8.0 (mg/ml)
4	1	drop	glycerol	10.0 (%(v/v))