1GXP

PhoB effector domain in complex with pho box DNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	110
Detector technology	CCD
Collection date	2001-04-15
Detector	QUANTUM
Spacegroup name	P 61
Unit cell lengths	74.106, 74.106, 289.682
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.500
R-factor	0.244 *
Rwork	0.244
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008 *
RMSD bond angle	1.469 *
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.600	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.098	0.338
Total number of observations	94255 *
Number of reflections	29719
<I/σ(I)>	5.4	2.1
Completeness [%]	95.8	76.4
Redundancy	3.2	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	20 *	pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	HEPES	100 (mM)	pH7.0
3	1	reservoir		1 (M)
4	1	reservoir	PEG6000	22 (%(w/v))