1GXP
PhoB effector domain in complex with pho box DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-04-15 |
| Detector | QUANTUM |
| Spacegroup name | P 61 |
| Unit cell lengths | 74.106, 74.106, 289.682 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.244 * |
| Rwork | 0.244 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.469 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.600 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.098 | 0.338 |
| Total number of observations | 94255 * | |
| Number of reflections | 29719 | |
| <I/σ(I)> | 5.4 | 2.1 |
| Completeness [%] | 95.8 | 76.4 |
| Redundancy | 3.2 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | 20 * | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 3 | 1 | reservoir | 1 (M) | ||
| 4 | 1 | reservoir | PEG6000 | 22 (%(w/v)) |
