1GXN

Family 10 polysaccharide lyase from Cellvibrio cellulosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	106.258, 55.137, 47.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.450 - 1.500
R-factor	0.131
Rwork	0.129
R-free	0.15700
Structure solution method	OTHER
RMSD bond length	0.010
RMSD bond angle	1.226
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.08)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.062	0.311
Number of reflections	45240
<I/σ(I)>	21.5	4.5
Completeness [%]	99.1	98.6
Redundancy	4.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	291 *	Charnock, S.J., (2001) Acta Crystallogr., D57, 1141. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	sodium MES		pH5.2
3	1	drop		200 (mM)
4	1	reservoir	PEG2000 MME	20 (%(w/v))