1GXM
Family 10 polysaccharide lyase from Cellvibrio cellulosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.670, 106.113, 55.365 |
Unit cell angles | 90.00, 91.95, 90.00 |
Refinement procedure
Resolution | 15.710 - 1.320 |
R-factor | 0.132 |
Rwork | 0.130 |
R-free | 0.16200 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.365 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.08) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.350 |
High resolution limit [Å] | 1.320 | 1.320 |
Rmerge | 0.058 | 0.329 |
Number of reflections | 112216 | |
<I/σ(I)> | 17.9 | 2.4 |
Completeness [%] | 86.8 | 35.4 |
Redundancy | 3.3 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | Charnock, S.J., (2001) Acta Crystallogr., D57, 1141. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | sodium MES | pH5.2 | |
3 | 1 | drop | 200 (mM) | ||
4 | 1 | reservoir | PEG2000 MME | 20 (%(w/v)) |