1GXL
SMC hinge domain from T. maritima with coiled coil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.400, 115.500, 68.960 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.252 * |
| Rwork | 0.252 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | P212121 CRYSTAL FORM |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.363 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.075 | 0.336 |
| Number of reflections | 18320 | |
| <I/σ(I)> | 16.3 | 2.9 |
| Completeness [%] | 95.1 | 92.6 |
| Redundancy | 5.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4.6 | 19 * | pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 3 | 1 | reservoir | iso-propanol | 30 (%) |
