1GXK

SMC hinge domain from T. maritima w/o coiled coil, P212121 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	58.900, 62.210, 225.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.000
R-factor	0.253 *
Rwork	0.253
R-free	0.29800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	P21 CRYSTAL FORM PDB ID 1GXJ
RMSD bond length	0.009
RMSD bond angle	1.332
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.160
High resolution limit [Å]	3.000	3.000
Rmerge	0.093	0.235
Number of reflections	13536
<I/σ(I)>	10.6	3.1
Completeness [%]	92.7	92.6
Redundancy	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.9 *	19 *	pH 9.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG2000 MME	15 (%)
2	1	reservoir	Tris	0.1 (M)	pH6.9
3	1	drop	protein	10 (mg/ml)