1GXA
BOVINE BETA-LACTOGLOBULIN COMPLEXED WITH RETINOL AND PALMITIC ACID, TRIGONAL LATTICE Z
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.636, 53.636, 112.027 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.350 |
| R-factor | 0.2186 |
| R-free | 0.30100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b0o |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.018 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 2.350 | |
| Rmerge | 0.043 | 0.254 |
| Total number of observations | 123391 * | |
| Number of reflections | 8186 | |
| <I/σ(I)> | 20.2 | |
| Completeness [%] | 99.2 | 97.2 |
| Redundancy | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 17 * | 17 DEG C, 8MUL BLG-RET-PLM COMPLEX 20MM TRIS, PH 8 + 8MUL NA CITRATE 1.25M, 0.1M HEPES, PH7.3, PER DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH8.0 |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | reservoir | sodium citrate | 1.25 (M) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.3 |
