1GX8
BOVINE BETA-LACTOGLOBULIN COMPLEXED WITH RETINOL, TRIGONAL LATTICE Z
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | RAYONIX MX340-HS |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 53.579, 53.579, 110.945 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.2101 |
R-free | 0.30600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b0o |
RMSD bond length | 0.005 * |
RMSD bond angle | 0.021 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.400 | |
Rmerge | 0.051 | 0.420 |
Total number of observations | 123384 * | |
Number of reflections | 7631 | |
<I/σ(I)> | 20.8 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 16.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 17 * | 17 DEG C, 8MUL BLG-RET COMPLEX 20MM TRIS, PH 8 + 8MUL NA CITRATE 1.25M, 0.1M HEPES, PH7.3 DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 20 (mM) | pH8.0 |
2 | 1 | drop | protein | 20 (mg/ml) | |
3 | 1 | reservoir | sodium citrate | 1.25 (M) | |
4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.3 |