1GX2
Recombinant horseradish peroxidase Phe209Ser complex with benzhydroxamic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.036, 62.298, 78.246 |
| Unit cell angles | 90.00, 104.27, 90.00 |
Refinement procedure
| Resolution | 37.920 - 2.200 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2atj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.920 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.106 | 0.391 |
| Number of reflections | 113464 | |
| Completeness [%] | 96.6 | 93.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.50 |
