1GX0

ALPHA-,1,3 GALACTOSYLTRANSFERASE - BETA-D-GALACTOSE COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-15
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	45.059, 94.277, 94.364
Unit cell angles	90.00, 99.09, 90.00

Refinement procedure

Resolution	24.000 * - 1.800
R-factor	0.189
Rwork	0.189
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4v
RMSD bond length	0.005 *
RMSD bond angle	23.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000 *	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.064	0.388
Total number of observations	280613 *
Number of reflections	72098 *
<I/σ(I)>	12	2.3
Completeness [%]	95.9	88.8
Redundancy	9	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	16 *	PEG6000, TRIS/HCL, pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MES-NaOH	20 (mM)	pH6.0
2	1	drop	glycerol	10 (%)
3	1	drop	UDP-Gal	10 (mM)
4	1	drop		0.1 (mM)
5	1	reservoir	PEG6000	5 (%)
6	1	reservoir	Tris-HCl	0.1 (M)	pH8.0