1GX0
ALPHA-,1,3 GALACTOSYLTRANSFERASE - BETA-D-GALACTOSE COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.059, 94.277, 94.364 |
Unit cell angles | 90.00, 99.09, 90.00 |
Refinement procedure
Resolution | 24.000 * - 1.800 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4v |
RMSD bond length | 0.005 * |
RMSD bond angle | 23.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.000 * | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.064 | 0.388 |
Total number of observations | 280613 * | |
Number of reflections | 72098 * | |
<I/σ(I)> | 12 | 2.3 |
Completeness [%] | 95.9 | 88.8 |
Redundancy | 9 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 16 * | PEG6000, TRIS/HCL, pH 8.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MES-NaOH | 20 (mM) | pH6.0 |
2 | 1 | drop | glycerol | 10 (%) | |
3 | 1 | drop | UDP-Gal | 10 (mM) | |
4 | 1 | drop | 0.1 (mM) | ||
5 | 1 | reservoir | PEG6000 | 5 (%) | |
6 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |