1GWY
Crystal structure of the water-soluble state of the pore-forming cytolysin Sticholysin II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2001-05-15 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.304, 119.735, 43.421 |
Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
Resolution | 10.000 * - 1.700* |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB CODE 1IAZ |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.300 * | 1.810 |
High resolution limit [Å] | 1.700 * | 1.710 |
Rmerge | 0.086 | 0.151 |
Number of reflections | 31889 | |
<I/σ(I)> | 5 | 4.6 |
Completeness [%] | 90.3 | 90.3 |
Redundancy | 2.1 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 20 (mM) | pH7.0 |
2 | 1 | drop | protein | 6-8 (mg/ml) | |
3 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
4 | 1 | reservoir | Tris | 0.1 (M) | pH7.5 |
5 | 1 | reservoir | PEG4000 | 25-30 (%(w/v)) |