1GWN

The crystal structure of the core domain of RhoE/Rnd3 - a constitutively activated small G protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-15
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	59.454, 59.454, 233.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.300 * - 2.100
R-factor	0.183 *
Rwork	0.183
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1CXZ CHAIN A
RMSD bond length	0.021
RMSD bond angle	1.920 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.800	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.079	0.343
Total number of observations	115552 *
Number of reflections	25276	3592 *
<I/σ(I)>	6.4	2.2
Completeness [%]	99.8	100
Redundancy	4.5	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	16 *	0.1M HEPES PH 7.5, 10% PROPAN-2-OL, 20% PEG 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)	pH7.5
3	1	reservoir	propanol-di-ol	10 (%)
4	1	reservoir	PEG4000	20 (%(w/v))