1GWN
The crystal structure of the core domain of RhoE/Rnd3 - a constitutively activated small G protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-15 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 59.454, 59.454, 233.024 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.300 * - 2.100 |
R-factor | 0.183 * |
Rwork | 0.183 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1CXZ CHAIN A |
RMSD bond length | 0.021 |
RMSD bond angle | 1.920 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.800 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.079 | 0.343 |
Total number of observations | 115552 * | |
Number of reflections | 25276 | 3592 * |
<I/σ(I)> | 6.4 | 2.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 16 * | 0.1M HEPES PH 7.5, 10% PROPAN-2-OL, 20% PEG 4000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
3 | 1 | reservoir | propanol-di-ol | 10 (%) | |
4 | 1 | reservoir | PEG4000 | 20 (%(w/v)) |