1GW8
quasi-atomic resolution model of bacteriophage PRD1 sus607 mutant, obtained by combined cryo-EM and X-ray crystallography.
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI/PHILIPS CM200FEG |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 21 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 13.3 |
| Software | EMfit,MRC IMAGE PROCESSING PACKAGE,SPIDER |
| Symmetry | I |
