1GW0
Crystal Structure of Laccase from Melanocarpus albomyces in Four Copper Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200HB |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.510, 72.300, 88.940 |
| Unit cell angles | 110.40, 95.23, 109.74 |
Refinement procedure
| Resolution | 100.000 - 2.400 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a65 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.092 | 0.280 |
| Number of reflections | 49561 | |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 95.0 | 86.7 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 25% PMME 2000, 0.2 M LITHIUM SULFATE, 0.1 M SODIUM ACETATE PH 4.2 |
