1GVZ
Prostate Specific Antigen (PSA) from stallion seminal plasma
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 72.668, 79.104, 45.783 |
Unit cell angles | 90.00, 98.21, 90.00 |
Refinement procedure
Resolution | 19.650 - 1.420 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.20900 * |
Structure solution method | MIRAS |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.606 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.470 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.074 | 0.487 |
Total number of observations | 490556 * | |
Number of reflections | 48282 | |
<I/σ(I)> | 17.9 | 2.5 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | other * | 6.5 | Carvalho, A.L., (2001) Acta Cryst., D57, 1180. * |