1GVY

Substrate distorsion by beta-mannanase from Pseudomonas cellulosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-15
Detector	ADSC CCD
Spacegroup name	P 41
Unit cell lengths	93.192, 93.192, 54.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.145
Rwork	0.144
R-free	0.17000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j9y
RMSD bond length	0.017
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.061	0.172
Number of reflections	50905
<I/σ(I)>	20.37	6.75
Completeness [%]	99.4	96.4
Redundancy	3.48	2.57

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		Hogg, D., (2001) J.Biol.Chem., 276, 31186. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG550 MME	26 (%)
2	1	reservoir	zinc sulfate	0.012 (M)
3	1	reservoir	MES	0.1 (M)