1GVL
Human prokallikrein 6 (hK6)/ prozyme/ proprotease M/ proneurosin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC/MAR RESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.000, 66.400, 71.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.200 - 1.800 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1npm |
RMSD bond length | 0.013 |
RMSD bond angle | 1.390 |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.600 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.095 | 0.436 |
Total number of observations | 64722 * | |
Number of reflections | 19445 | |
<I/σ(I)> | 4.6 | 1.7 |
Completeness [%] | 94.0 | 90.1 |
Redundancy | 3.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 30 (%) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | drop | Tris-HCl | 10 (mM) | pH8.5 |
6 | 1 | drop | benzamidine | 10 (mM) |