1GVI

Thermus maltogenic amylase in complex with beta-CD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	P 61
Unit cell lengths	119.090, 119.090, 270.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.300
R-factor	0.192
Rwork	0.192
R-free	0.24300
Structure solution method	DIRECT METHODS
RMSD bond length	0.008
RMSD bond angle	1.481
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.300
Rmerge	0.107 *	0.260 *
Total number of observations	271781 *
Number of reflections	32689 *
Completeness [%]	89.3 *	81.4 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8 *	22 *	Kim, J.S., (1999) J. Biol. Chem., 274, 26279. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
10	1	reservoir	ethanol	4 (%(v/v))
2	1	drop	maleate	16 (mM)	pH6.8
3	1	drop	lithium sulfate	0.1 (M)
4	1	drop	ammonium sulfate	0.06 (M)
5	1	drop	sodium citrate	0.02 (M)
6	1	drop	ethanol	0.8 (%(v/v))
7	1	reservoir	lithium sulfate	0.5 (M)
8	1	reservoir	ammonium sulfate	0.3 (M)
9	1	reservoir	sodium citrate	0.1 (M)	pH5.6