1GVG
Crystal Structure of Clavaminate Synthase with Nitric Oxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.270, 69.520, 70.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.540 |
| R-factor | 0.1842 |
| Rwork | 0.184 |
| R-free | 0.20740 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ds0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.054 | 0.298 |
| Total number of observations | 301822 * | |
| Number of reflections | 46381 | 4038 * |
| <I/σ(I)> | 29.1 | 3.8 |
| Completeness [%] | 97.7 | 86 |
| Redundancy | 13.2 | 4.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * | pH 7.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 100 (mM) | pH7.5 |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | glycerol | 10 (%) |
