1GV9
p58/ERGIC-53
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 110 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 49.043, 85.407, 127.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.460 |
| R-factor | 0.191 * |
| Rwork | 0.191 |
| R-free | 0.21100 * |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.490 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 1.460 | 1.460 * |
| Rmerge | 0.041 | 0.119 * |
| Number of reflections | 44706 | |
| <I/σ(I)> | 22.2 | |
| Completeness [%] | 95.6 | 84.6 * |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.25 | pH 7.25 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.25 |
| 5 | 1 | reservoir | 1.6 (M) | ||
| 6 | 1 | reservoir | EDTA | 10 (mM) |
