1GV8

18 kDa fragment of N-II domain of duck ovotransferrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1993-01-15
Detector	RIGAKU RAXIS II
Spacegroup name	P 31
Unit cell lengths	41.390, 41.390, 81.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.220 - 1.950
R-factor	0.171
Rwork	0.169
R-free	0.21800
Structure solution method	OTHER
RMSD bond length	0.021 *
RMSD bond angle	1.930 *
Data reduction software	MOSFLM
Data scaling software	Agrovata
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.220	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.055	0.103
Total number of observations	33700 *
Number of reflections	11119
<I/σ(I)>	6.6	5.8
Completeness [%]	97.6	94.5
Redundancy	3.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	277	SITTING DROP AT PH 7.8, 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)
2	1	drop	protein	20-40 (mg/ml)
3	1	reservoir	methanepentanediol	23 (%)