1GV5

CRYSTAL STRUCTURE OF C-MYB R2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MAC SCIENCE M06XHF22
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-10-29
Detector	MAC Science DIP-2030
Spacegroup name	P 21 21 21
Unit cell lengths	28.832, 40.182, 49.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.260 - 1.580
R-factor	0.178
Rwork	0.178
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mbg
RMSD bond length	0.004
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.640
High resolution limit [Å]	1.580	1.580
Rmerge	0.059	0.329
Number of reflections	8207
<I/σ(I)>	42.736	5.285
Completeness [%]	99.7	98.9
Redundancy	11.16	6.17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8	297	1.65 M SODIUM CITRATE PH 6.8, PROTEIN CONCENTRATION 10 MG/ML PLUS 10 MM DTT, TEMPERATURE 297 K