1GV3
The 2.0 Angstrom resolution structure of the catalytic portion of a cyanobacterial membrane-bound manganese superoxide dismutase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 2001-08-15 |
Detector | MARRESEARCH |
Spacegroup name | H 3 |
Unit cell lengths | 151.320, 151.320, 69.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.570 * - 2.030* |
R-factor | 0.188 |
Rwork | 0.188 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mng |
RMSD bond length | 0.006 |
RMSD bond angle | 1.248 |
Data reduction software | MOSFLM |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.570 |
High resolution limit [Å] | 2.030 |
Total number of observations | 124807 * |
Number of reflections | 35333 |
Completeness [%] | 93.9 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
4 | 1 | reservoir | isopropanol | 5 (%(v/v)) |