1GV3

The 2.0 Angstrom resolution structure of the catalytic portion of a cyanobacterial membrane-bound manganese superoxide dismutase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	2001-08-15
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	151.320, 151.320, 69.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.570 * - 2.030 *
R-factor	0.188
Rwork	0.188
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mng
RMSD bond length	0.006
RMSD bond angle	1.248
Data reduction software	MOSFLM
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.570
High resolution limit [Å]	2.030
Total number of observations	124807 *
Number of reflections	35333
Completeness [%]	93.9
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5		pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH7.5
3	1	reservoir	ammonium sulfate	2.0 (M)
4	1	reservoir	isopropanol	5 (%(v/v))