1GV2

CRYSTAL STRUCTURE OF C-MYB R2R3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-05-29
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	38.722, 28.250, 44.129
Unit cell angles	90.00, 90.87, 90.00

Refinement procedure

Resolution	44.120 - 1.680
R-factor	0.193
Rwork	0.193
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1MBG AND 1MBJ
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.740
High resolution limit [Å]	1.680	1.680
Rmerge	0.100	0.446
Number of reflections	11033
<I/σ(I)>	12.601	1.904
Completeness [%]	99.1	97.9
Redundancy	4.244	3.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8		1.6-1.7 M SODIUM CITRATE PH 6.8, PROTEIN CONCENTRATION 15 MG/ML, CRYSTAL WAS GROWN BY REPEATED MACROSEEDING, TRANSFORMED TO LOW HUMIDITY FORM AND FLASH COOLED, 1-2% V/V OF GLYCEROL WAS ADDED TO SODIUM CITRATE TO PREVENT THE CRYSTAL CRACKING DURING THE FLASH COOLING