1GUU
CRYSTAL STRUCTURE OF C-MYB R1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-27 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.273, 35.303, 24.039 |
| Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
| Resolution | 19.850 - 1.600 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mbe |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.077 | 0.204 |
| Number of reflections | 5574 | |
| <I/σ(I)> | 14.653 | 2.279 |
| Completeness [%] | 93.9 | 72.5 |
| Redundancy | 2.959 | 2.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 1.6-1.7 M SODIUM CITRATE PH 6.8, PROTEIN CONCENTRATION 15 MG/ML, CRYSTAL WAS TRANSFORMED TO LOW HUMIDITY FORM AND FLASH COOLED, 1-2% V/V OF GLYCEROL WAS ADDED TO PREVENT THE CRYSTAL CRACKING DURING THE FLASH COOLING |
