1GUT

MopII from Clostridium pasteurianum (apo2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-07-15
Detector	RIGAKU IMAGE PLATE
Spacegroup name	C 1 2 1
Unit cell lengths	78.961, 82.328, 57.031
Unit cell angles	90.00, 93.44, 90.00

Refinement procedure

Resolution	25.000 - 1.500
R-factor	0.181
Rwork	0.178
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gug
RMSD bond length	0.011
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.067	0.248
Total number of observations	1132889 *
Number of reflections	55394
<I/σ(I)>	18.9	4.2
Completeness [%]	95.5	85.4
Redundancy	4	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	293 *	Harrison, J.A., (2001) Acta Crystallogr., D57, 1715. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir		2 (M)
3	1	reservoir	PEG6000	10 (%(w/v))