1GUS
MopII from Clostridium pasteurianum (apo1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Collection date | 2001-05-15 |
Detector | RIGAKU R-AXIS IV |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.080, 82.400, 56.820 |
Unit cell angles | 90.00, 93.23, 90.00 |
Refinement procedure
Resolution | 23.770 - 1.800 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gug |
RMSD bond length | 0.019 |
RMSD bond angle | 2.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.041 | 0.280 |
Total number of observations | 124432 * | |
Number of reflections | 33112 | |
<I/σ(I)> | 16.8 | 2.6 |
Completeness [%] | 97.6 | 93.9 |
Redundancy | 1.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 293 * | Harrison, J.A., (2001) Acta Crystallogr., D57, 1715. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | reservoir | 2 (M) | ||
3 | 1 | reservoir | PEG6000 | 10 (%(w/v)) |