1GUO
MopII from Clostridium pasteurianum complexed with molybdate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-07-15 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 55.144, 77.764, 93.494 |
Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
Resolution | 29.880 - 2.500 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gug |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.050 | 0.259 |
Total number of observations | 173881 * | |
Number of reflections | 10097 | |
<I/σ(I)> | 27.7 | 4.4 |
Completeness [%] | 98.3 | 89.2 |
Redundancy | 4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 293 * | Harrison, J.A., (2001) Acta Crystallogr., D57, 1715. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 8 (mM) | fivefold molar excess relative to MopII | |
2 | 1 | reservoir | HEPES | 95 (mM) | pH7.5 |
3 | 1 | reservoir | PEG400 | 26.6 (%(w/v)) | |
4 | 1 | reservoir | glycerol | 5 (%(w/v)) | |
5 | 1 | reservoir | 190 (mM) | ||
6 | 1 | drop | protein | 30 (mg/ml) |