1GUI
CBM4 structure and function
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-15 |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 105.117, 105.117, 49.636 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.173 |
Rwork | 0.172 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gu3 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.593 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BEAST |
Refinement software | REFMAC (5.1.07) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.044 | 0.281 |
Number of reflections | 25221 | |
<I/σ(I)> | 34 | 7.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 2.1 * | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | 2.0M AMMONIUM SULFATE 100MM SODIUM ACETATE PH4.6, pH 4.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium acetate | 0.1 (M) | |
2 | 1 | reservoir | ammonium sulfate | 2.0 (M) |