1GUH
Structure determination and refinement of human alpha class glutathione transferase A1-1, and a comparison with the MU and PI class enzymes
Experimental procedure
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.800, 95.400, 105.200 |
Unit cell angles | 90.00, 92.40, 90.00 |
Refinement procedure
Resolution | 7.500 * - 2.600 |
R-factor | 0.229 |
Rwork | 0.229 |
RMSD bond length | 0.011 |
RMSD bond angle | 2.800 * |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.600 * |
Rmerge | 0.053 * |
Total number of observations | 46733 * |
Number of reflections | 25135 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 20 * | referred to J.Mol.Biol. 208.369-370 1989 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 0.005ml | |
2 | 1 | drop | protein | 35 (mg/ml) | |
3 | 1 | drop | ligand | 2.5 (mM) | |
4 | 1 | drop | bistrispropane | 50 (mM) | |
5 | 1 | drop | 2-mercaptoethanol | 1 (%(v/v)) | |
6 | 1 | drop | PEG2000 | 20 (%(w/v)) | |
7 | 1 | reservoir | bistrispropane | 50 (mM) | |
8 | 1 | reservoir | 2-mercaptoethanol | 1 (%(v/v)) | |
9 | 1 | reservoir | PEG2000 | 20 (%(w/v)) |