1GUA
HUMAN RAP1A, RESIDUES 1-167, DOUBLE MUTANT (E30D,K31E) COMPLEXED WITH GPPNHP AND THE RAS-BINDING-DOMAIN OF HUMAN C-RAF1, RESIDUES 51-131
Experimental procedure
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.500, 71.800, 100.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.940 |
| Data reduction software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.041 * |
| Number of reflections | 26720 * |
| Completeness [%] | 98.0 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 * | 19 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 11 (%) | |
| 10 | 1 | drop | 5 (mM) | ||
| 11 | 1 | drop | calcium acetate | 20-50 (mM) | |
| 2 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 80 (mM) | |
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | calcium acetate | 20-50 (mM) | |
| 6 | 1 | drop | Rap/RafRBD | 15 (mg/ml) | |
| 7 | 1 | drop | PEG4000 | 11 (%) | |
| 8 | 1 | drop | dithiothreitol | 2 (mM) | |
| 9 | 1 | drop | Tris-HCl | 80 (mM) |
