1GU4
Crystal structure of C/EBPBETA BZIP homodimer bound to a high affinity DNA fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 2001-10-12 |
Detector | RIGAKU RAXIS V |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.947, 112.326, 74.401 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.950 - 1.800 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gtw |
RMSD bond length | 0.004 |
RMSD bond angle | 0.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.094 | 0.455 |
Number of reflections | 42716 | |
<I/σ(I)> | 21.7331 | 2.079 |
Completeness [%] | 99.4 | 98.3 |
Redundancy | 5.452 | 3.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.2 M POTASSIUM CHLORIDE, 0.01 M MAGNESIUM SULFATE, 10.0% V/V PEG 400, 0.05 M MES BUFFER PH 6.0, PROTEIN-DNA COMPLEX CONCENTRATION WAS 12 MG/ML AND CONTAINS 0.01 M DTT, PROTEIN:DNA RATIO WAS 1:1.2. FOR CRYOPROTECTION THE CONCENTRATION OF PEG 400 WAS ADJUSTED TO 36% V/V |