1GU2
Crystal structure of oxidized cytochrome c'' from Methylophilus methylotrophus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.537, 74.115, 78.388 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.190 |
| R-free | 0.16700 * |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.235 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.260 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.051 | 0.480 |
| Number of reflections | 109062 | |
| <I/σ(I)> | 7 | 1.95 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.5 (M) | |
| 2 | 1 | reservoir | glycerol | 20 (%) | |
| 3 | 1 | reservoir | 2.5 (mM) | ||
| 4 | 1 | reservoir | Tris aminomethane maleate | 100 (mM) | pH7.0 |
