1GTW

crystal structure of C/EBPbeta bZip homodimer bound to a DNA fragment from the tom-1A promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-12-11
Detector	RIGAKU IMAGE PLATE RAXIS IV
Spacegroup name	C 2 2 21
Unit cell lengths	100.938, 112.533, 74.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.870 - 1.850
R-factor	0.231
Rwork	0.231
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h8a
RMSD bond length	0.004
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.052	0.430
Number of reflections	44759
<I/σ(I)>	21.0506	1.343
Completeness [%]	95.8	82.9
Redundancy	4.008	2.43

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.2 M POTASSIUM CHLORIDE, 0.1 M MAGNESIUM ACETATE, 10.0% W/V PEG 8000, 0.05 M SODIUM CACODYLATE CACODYLATE BUFFER PH 6.5, PROTEIN-DNA COMPLEX SOLUTION CONTAINS 0.01 M DTT AND 0.005 M NAN3, PROTEIN:DNA RATIO IS 1:1.2